Recently, cerium oxide nanoparticles (CeO 2 NPs) have attracted great attentions due to their unique redox properties that were extensively applied in energy, catalysis, and biomedical fields.
#Oh radical xps peak series
Consequently, it is an important and urgent problem to control the concentration of reactive oxygen free radicals in living organisms and keep them in a suitable range, which is also basic to decrease series of oxidative abnormalities. Thus, oxidative stress plays a key role in the pathogenesis of macro vascular diseases, cancer, and some other nervous system diseases. The highly reactive ROS have a tendency to produce free radicals (including superoxide (O 2 −), HO 2ˋ and hydroxyl ( ˋOH)) which cause damage to macromolecules in the organism, such as proteins and nucleic acids, whereas overproduction of ROS makes a critical difference in sustained oxidative damage, leading to significant destructions of cellular structures and functions, such as inflammatory infiltrates, the damage of cell membranes and DNA, and even increasing cell death. Oxidative stress has been found in many clinical diseases where ROS has much higher expression than elimination of antioxidant enzymes, which finally is defined as an imbalance between oxidative and anti-oxidative performances. These findings suggest that Ce-doped CNPs as novel ROS scavengers may provide a potential therapeutic prospect in treating diseases associated with oxidative stress.Ĭontinuous and deregulated inflammation is considered to be a common step of numerous pathological processes. Furthermore, flow cytometry and live-dead staining results indicated that Ce-doped CNPs protected cells from H 2O 2-induced damage in a dose-dependent effect, which provided a direct evidence for anti-oxidative performance. Using H 2O 2-induced reactive oxygen species (ROS) as model, Ce-doped CNPs showed highly hydroxyl radical scavenging capability. The CCK-8 assay demonstrated that Ce-doped CNPs possessed favorable biocompatibility and negligible cytotoxicity. XPS and FTIR results revealed the presence of hydrophilic group on the surface of Ce-doped CNPs, which resulted in excellent dispersity in water. The resultant Ce-doped CNPs exhibited uniform and ultrasmall morphology with an average size of 14.7 nm. In this study, a simple, mild, and green approach was developed to synthesize cerium-doped carbonaceous nanoparticles (Ce-doped CNPs) using bio-mineralization of bull serum albumin (BSA) as precursor. Cerium oxide nanoparticles recently have received extensive attention in biomedical applications due to their excellent anti-oxidation performance.